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Objective:To study the effects of insulin and berberine on osseointegration of titanium implant in type Ⅱ diabetic rats.Methods:10 out of 53 SD rats were randomly selected and selected into H group(healthy group)(n =10).The remaining rats were treated with high glucose diet plus low dose of streptozotocin(STZ) and 40 rats were induced into the models of type Ⅱ diabeties.The 40 dibetic rats were randomly divided into group B(berberine),group Ⅰ(insulin),group IB(insulin + berberine) and group N(without treatment).2 pure titanium implants were implanted into the bilateral femoral metaphysis of each rat.Mter 7,32,38,66 and 101 d,the body weight(BW) and fasting blood glucose(GLU) were measured.After 10 week,the rats were sacrifice,femoral metaphysis samples were subjected to Micro-CT scan,VG staining and implant pull-out test.Hard tissue slices were observed;bone volume fraction(BV/TV),Liang Houdu (Tb.Th),trabecular bone trabecular number(Tb.N),Liang Jianxi (Tb.Sp),trabecular bone trabecular bone volume (TBV),bone contact ratio(BIC) and the maximum pull-out strength (MPF) were measured.Results:There was no significant difference of BW and GLU between IB group and H group(P ＞ 0.05),but the BW and GLU of IB group was better than those of Ⅰ group and B group,Ⅰ group and B group were better than those of group N (P ＜ 0.05).BV/TV,Tb.Th,Tb.N,Tb.Sp,TBV,BIC and MPF of IB group were not statistically different from those of H group(P ＞0.05);but the indexes of IB group were higher than those of Ⅰ group and B group,those of Ⅰ group and B group were higher than those of N group (P ＜ 0.05).Conclusion:The combination of insulin and berberine can effectively reduce blood glucose and can promote osseointegration of titanium implants in type Ⅱ diabetic rats.
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Objective@#To evaluate the effect of sandblasting on bending strength and subcritical crack growth (SCG) under cyclic loading of yttria-stabilized tetragonal zirconia polycrystal (Y-TZP) ceramics.@*Methods@#After being polished, sixty bar-shaped specimens of Y-TZP (Wieland zirconia ceramics) were assigned to two groups (n=30) according to the random number table, the sandblasting group (SG) which was treated with sandblasting and the control group (CG) which remained untouched. In each group, half of the specimens (n=15) were subjected to bending strength test, and the results were examined by Weibull statistics and analyzed with ANOVA. The other 15 specimens in each group were subjected to fatigue tests. The results were examined by Weibull statistics and subcritical crack propagation rates were calculated.@*Results@#The bending strengths of SG and CG were (1 291±133) and (1 140±124) MPa (F=10.117, P=0.004), and the Weibull modules of the two groups were 11.06 and 10.64 respectively. The crack growth rate of SCG of SG was lower than that of CG under the same cyclic loading.@*Conclusions@#Proper sandblasting on Y-TZP ceramic can increase its bending strength and resistance to SCG.
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Objective To compare the results of thrombelastography (TEG) and the conventional coagulability test in patients with sepsis, and to discuss the value of TEG in monitoring blood coagulation dysfunction in patients with sepsis. Methods The clinical data of 92 adult patients with sepsis admitted to Department of Critical Care Medicine of the First Affiliated Hospital of Guangxi Medical University were retrospectively analyzed. The patients were divided into sequential organ failure assessment (SOFA) score ≥ 12 group (n = 47) and SOFA 0.05; K value (minutes): 4.2 (3.4, 7.1) vs. 1.5 (1.3, 1.8), P < 0.01]. The α angle, maximum amplitude (MA) and coagulation index (CI) in SOFA < 12 group were higher than those of the non-sepsis group [α angle (° ): 73.3 (68.5, 74.7) vs. 66.8 (62.2, 69.0), P < 0.01; MA (mm): 71.7 (61.9, 73.3) vs. 60.3 (58.2, 63.8), P < 0.01; CI: 3.1 (-0.1, 3.9) vs. 0.9 (-0.4, 1.3), P < 0.05], while they were lower in SOFA ≥ 12 group than those of the non-sepsis group [α angle (° ): 48.1 (36.6, 53.0) vs. 66.8 (62.2, 69.0), P < 0.01; MA (mm): 37.8 (30.0, 45.7) vs. 60.3 (58.2, 63.8), P < 0.01; CI: -5.6 (-8.4, -3.6) vs. 0.9 (-0.4, 1.3), P < 0.01]. The above results indicated that TEG could distinguish quickly the hypercoagulability and hypocoagulability status in septic patients. PCT in non-sepsis, SOFA < 12, and SOFA ≥ 12 groups were 0.27 (0.05, 1.80), 0.68 (0.10, 10.00), 41.10 (4.24, 100.00) μg/L respectively (Z = 195.475, P = 0.000), which indicate the severity of infectious disease. Correlation analysis results showed that SOFA score was negatively correlated with PLT, α angle, MA, and CI (r value was -0.853, -0.833, -0.881, and -0.859, respectively, all P = 0.000), and it was positively correlated with activated partial thromboplastin time (APTT), D-dimer, R value, K value, and PCT (r value was 0.381, 0.561, 0.587, 0.831, 0.775, respectively, P < 0.05 or P < 0.01), and non correlations was founded with PT, fibrinogen (FBG), and INR (r1 = 0.211, P1 = 0.233; r2 = -0.252, P2 = 0.142; r3 = 0.248, P3 = 0.148). Conclusions TEG can effectively monitor the change in coagulation in patients with sepsis, and distinguish the hypercoagulable and hypocoagulable state. TEG may be a valuable tool to evaluate degree and risk of sepsis objectively.
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Objective:To evaluate the reliability of flexural strength and subcritical crack growth(SCG)of WL and HT zirconia ce-ramics under cyclic loading.Methods:Bar-shaped speciments of WL(n =30)and HT(n =30)were fabricated and loaded in three-point bending and cyclically fatigued.The flexural strength and the number of cycles to fracture were obtained from three-point flexural test and cyclic fatigue test respectively.Data were examined using Weibull statistics.Pairs law was used to analysize the subcritical crack growth(SCG),the behavior of which was described in terms of crack velocity as a function of maximum stress intensity factor (KImax ).Results:The Weibull module(m)of WL and HT was 10.64 and 12.04,the stress corrosion cracking susceptive indexes(n) 23.71 and 32.87,the SCG 90% and 76.7%,the KImax 49.6% and 50.9% of its maximum stress intensity factor(KIC ),respectively. Conclusion:WL and HT produce pronounced SCG.The minimum fatigue crack growth rate of HT is lower than that of WL(νHT <νWL ).
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This article systematically introduces the basic conception,classification and physiological significance of anterior guidance for anterior prostheses.A customized incisal guidance table was introduced to record and transfer the individual value of the patient to a virtual full-adjustable articulator system.The clinic effects of the individual value and average value were also compared.
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Objective:To compare the adaption of cobalt-chromium prostheses fabricated by selective laser melting(SLM)and conven-tional lost wax technique(LW)method.Methods:Cobalt-chromium crowns and three-unit fixed dental prostheses(FDPs)were fabrica-ted by selective laser melting(SLM)or by conventional lost wax technique(LW)respectively(n =5).Marginal fit and internal fit were examined using a light-body silicone.After setting,each silicon film was cut into 2 parts and the thickness of silicon layer was measured at 1 00 ×magnification using a digital microscope,the data of marginal gap(MG)and internal gap(IG)were statistically analysed by ANOVA and SPSS statistical package version 1 7.0.Results:The MG and IG(μm)of cobalt-chromium alloy crowns in EOS M270 group were 42.28 ±1 1 .58 and 1 25.75 ±47.67,in EOS M280 group 46.54 ±8.4 and 1 28.22 ±54.1 8,in LW group 48.66 ±1 2.08 and 1 35.37 ±46.89.The MG and IG(μm)of three-unit FDPs in EOS M270 group were 40.55 ±1 0.04 and 1 33.09 ±39.80,in EOS M280 group 45.36 ±1 0 and 1 38.94 ±50.61 ,in LW group 54.24 ±1 2.04 and 1 51 .87 ±61 .94(P <0.05),respectively.Conclu-sion:The marginal fit of the SLMgroup is superior to that of the LW group and significantly smaller than 1 20 μm of clinical generally accepted standards.SLMsystem can satisfy the clinical requirements.
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Objective To compare the ventilatory effects between three-way laryngeal mask airway (TLMA)and tracheal catheter (TC) on hemodynamics, respiratory function and stress responses on patients during bronchoalveolar lavage (BAL). Method Forty patients scheduled for BAL under general anesthesia were divided (stratified sampling) into either TLMA group (group T,n = 20) or TC group (group C, n = 20) according to the stratified sampling principle. SpO2, SBP, DBP and HR were measured in 5 min after entering the operating theater (To), just before inserting TLMA or TC(T1), immediately after inserting TLMA or TC(T2) ,3 min(T3), 5 min(T4), 10 min(T5)after mechanical ventilation, 10 min(T6),20 min(T7), 30 min(T8)during the course of BAL,immediately after extubating TLMA or TC (T9)and 3 min after extubating TLMA or TC (T10). The tidal volume (VT), peak inspiratory airway pressure (Ppeak) and end expiratory CO2 pressure(PETCO2)were recorded at T2,T4,T6,T7, T8, T10. The venous blood samples were taken at T0, T2, T3, T4, T6, T9, T10 for the measurements of epinephrine(AE), norepinephrine(NE)and dopamine (DA) levels with high performance liquid chromatography.Data were dealt with SPSS version 10.0 statistic software. The variables of hemodynamics and stress responses were analyzed with ANOVA of repeating test data. P ＜ 0.05 means the difference in statistical significance. Results In group C, SBP, DBP and HR were significantly higher than those in group T at T2 ,T3 ,T9 (P ＜ 0.05). In group C, the levels of Ppeak were significantly higher than those in group T at T6 ,T7 ,T8 (P ＜ 0.05), and the concentrations of AE, NE and DA were also significantly higher in group C than those in group T at T2, T3 and T9 (P ＜0.05). Conclusions Ventilation with TLMA in patients during BAL is better than TC in respects of keeping stable ventilation, stable hemodynamics and producing less stress responses.
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Full ceramic prosthesis is becoming the main prosthetic development direction. As an important kind of full ceramic prosthesis, full ceramic veneer has not only natural cosmetic appearance and perfect biocompatibility, but also can preserve tooth structure as much as possible, which would be more easily accepted by patients for cosmetic prosthesis. Many methods, such as slip casting, die casting and CAD/CAM technology are available to make full ceramic veneer. All the methods have their own advantages and disadvantages. Slip casting is easy to be developed widely with low cost, so be used in practical work. In this article, the working process of full ceramic veneer, especially manufacture of removable dies, duplication of refractory models, were introduced, which was supposed to be beneficial for the technicians.
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A hydrostatic compression device has been designed to study the effects of intermittent or continuous compressive forces on cells by trying to mimic in vivo forces. The model system is composed of two pistons that deliver and release compressed air under control of a motor to form an enclosed tissue culture chamber housing numerous tissues culture dishes. The continuous compressive forces and intermittent compressive forces are within 100-300 KPa and 0-30 KPa respectively. The compressive force is uniform in the system and can also result in the generation of shear forces along the interface between the cells and culture substratum, so the hydrostatic compression device can be used to study the characteristics of in vitro cells loading models.
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Humans , Biomechanical Phenomena , Cell Culture Techniques , Methods , Compressive Strength , Computer-Aided Design , Equipment Design , Shear Strength , Stress, MechanicalABSTRACT
Both with the advantages of fixed and removable dentures,the attachment prostheses is an important component of prosthodontics.At present,the resin base for the removable part of attachment denture was majorly made with traditional methods such as cartoning,resin-filling,heat treatment.But the produced fixed part of attachment denture was easy to be deformed or broken off during such processes.Here a method were introduced to produce resin base with injection moulding,which could avoid the occurrence of the mistakes of traditional methods and simplify the process.
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0.05).Conclusions:Laser welding joint gap at 0.00~1.00 mm does not affect the mechanical property of laser-welded titanium.
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Objective: To study the stress in bone implant interface, dentin and periodontal ligament while an endodontic endosseous implant is placed through a centeral incisor with periodontal compromises. Methods: Two dimension finite element analysis method was used and finite element analysis (FEA) model of the Chinese adult central incisor with II degree looseness according the data from literature was established. A load of 100 N was applied vertically or at 45?to vertical. The stress in bone implant interface, dentin and periodontal ligament was measured and analysed with Super sap(1991 edition) FEA software in a IBM PC(PIII 550).Results: The mobility of the tooth was decreased by endodontic endosseous implant with periodontal compromises, and lower stress was observed at the alveolar crest and periodontal membrane after implantation with inclined load. High stress concentration on the implant/bone interface was produced below the canal orifice and implant apex, and at root apex within dentin.Conclusion: Endodontic endosseous implant can be used to stabilize the tooth with periodontal compromises, and to improve the stress distribution around the root.
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Objective: To evaluate the function of dentin sialophosphoprotein (DSPP) during mouse teeth development. Methods:Expression of DSPP mRNA in the first molar of Balb/c mice at different developmental stages was detected by in situ hybridization. Resullts: DSPP mRNA in odontoblasts was detected in the samples at middle bell stage and ran through the later stages. In addition, it was detected in ameloblasts as well as in preameloblasts. Conclusion: DSPP expresses with temporospatial characters. It may play an important role in tooth morphogenesis
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OBJECTIVE:To study whether there is interaction between ambroxol hydrochloride and amino?phylline.METHODS:16volunteer were randomly divided into trial group and control group.The blood concentration of amin_ ophylline was determinated with HPLC method,then the main parameters of pharmacokinetics of aminophylline were calculated with3p97software.RESULTS:The main parameters of pharmacokinetics of aminophylline in trial and control group were:K e (0.062?0.023)/h and(0.0905?0.013)/h;V(22.83?7.85)and(23.27?3.78)L;T 1/2 (12.51?4.2)and(7.77?0.89)h;AUC 0～21 (426.59?186.92)and(245.74?48.6)(mg?h)/L;CL(s)(23.17?9.83)and(34.84?6.00)ml/min respective?ly.CONCLUSION:Although ambroxol hydrochloride did not influence the distribution of aminophylline,but it could prolong the half time of aminophylline and increase the stagnation time of aminophylline in vivo.